Image of the Month (2010-4)
Use of CK5/6 immunohistochemistry to investigate possible invasion in DCIS
1cm mass in left breast; clinical invasive cancer; core biopsy
Medium power view showing area suspicious of invasion
High power detail of above
CK5/6 staining of focus of normal breast tissue showing positive staining of myoepithelial layer. This is an internal control and is the best way to judge the pattern of immunostaining elsewhere in the section.
Area of unequivocal DCIS showing CK5/6 positive bounding layer
Area suspicious of invasion - 'Mouse-over'to see intact CK5/6 positive bounding layer confirming in situ disease.